摘 要: 探讨抗氧化剂对砷诱导人正常膀胱上皮细胞 HIF-1α 表达的影响。 方法 人膀胱上皮细胞(SV-HUC-1)暴露于0、05、1、2、4、8、10 μmol/L亚砷酸钠(NaAsO2)24 h,RT-PCR检测 HIF-1α 的mRNA水平;选择4和10 μmol/L剂量组,分别处理4、12、24、48、72 h,再以RT-PCR法检测 HIF-1α 的mRNA水平;SV-HUC-1细胞同时暴露于NaAsO2和抗氧化剂 N-乙酰半胱氨酸(NAC)、维生素C(VC),检测HIF-1α的蛋白含量。 结果 经砷处理后,SV-HUC-1细胞 HIF-1α 的mRNA含量显著升高(P<0.05),并与染砷剂量呈显著剂量-反应关系;抗氧化剂NAC和VC均显著降低了HIF-1α的蛋白水平。 结论 砷能诱导人正常膀胱上皮细胞 HIF-1α 表达增加,抗氧化剂NAC和VC对砷诱导的HIF-1α表达具有显著的抑制作用。 |
关键词: 亚砷酸钠 膀胱癌 缺氧诱导因子1α(HIF-1α) 抗氧化剂 乙酰半胱氨酸(NAC) 维生素C |
中图分类号: R994.6
文献标识码:
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基金项目: 国家自然科学基金(编号:81072244,81373023) |
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Effect of antioxidants on arsenic-induced HIF-1α expression in human urothelial cells |
WANG Fei,LIU Jie yu,LI Meng dan,XI Shu hua
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Program of Geochemical Disease,Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning,School of Public Health,China Medical University,Shenyang 110122,China
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Abstract: To explore the effect of antioxidants to arsenic induced HIF-1αexpression in human urothelial cells. Methods SV-HUC-1 cells were treated with NaAsO2 (0、0.5、1、2、4、8、10 μmol/L,respectively)for 24 h, their mRNA expression levels of HIF-1αwere analyzed using RT-PCR,βactin as a loading control. Then SV-HUC-1 cells were treated with 4 and 10 μmol/L with NaAsO2 together for 4、12、24、48 and 72 h respectively,the cells expression levels of HIF-1αwere analyzed using RT-PCR as well and the protein expression levels of HIF-1α were measured by western blot. Results The mRNA expressions of HIF-1α in As treated cells were increased significantly at the concentration of 10 μmol/L NaAsO2(P<0.05);while the antioxidants(NAC and VC)could decrease the protein expression of HIF-1α . Conclusion The results suggest that antioxidants may decrease the risen expression of HIF-1α induced by As. |
Keywords: arsenic bladder cancer hypoxia induced factor 1α(HIF-1α) antioxidants acetyl cysteine(NAC) Vit C |