摘 要: 目的 观察软肝冲剂对四氯化碳(CCl4) 诱导的小鼠肝纤维化模型的保护作用。方法 将60 只C57B/ L小鼠随机分为空白对照组、模型组、秋水仙碱对照组、软肝冲剂低剂量组和软肝冲剂高剂量组, 每组12 只。空白对照组腹腔注射100%花生油1 ml/ kg, 每周2 次, 共10 周; 其余各组均给予腹腔注射含20%CCl4 的花生油1 ml/ kg, 每周2 次, 共10 周造模; 第3 周起秋水仙碱对照组予秋水仙碱0. 1 mg/ (kg•d) 灌胃, 软肝冲剂低、高剂量组分别予软肝冲剂1 g/ (kg•d)、5 g/ (kg•d) 灌胃。在第10 周通过摘除眼球取血, 检测小鼠血清中透明质酸(HA)、层黏连蛋白(LN)、三型前胶原(PC Ⅲ)、四型胶原(Ⅳ⁃C)、丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST) 含量, 检测肝脏组织中超氧化物歧化酶(SOD) 和丙二醛(MDA) 的含量, 计算肝脏指数, 观察小鼠肝脏的HE 染色病理组织学改变。结果 模型组小鼠血清中HA、LN、PCⅢ、Ⅳ⁃C、ALT 和AST 含量分别为(480. 11±62. 12) μg/ L、(411. 65±30. 65) μg/ L、(454. 12±41. 81) μg/ L、(311. 15±31. 61) μg/ L、(140. 4±9. 8) U/ L 和(198. 2±9. 7) U/ L, 均明显高于其它各组(P<0. 05); 肝脏组织中SOD [ (122. 53±7. 68) U/ mg] 低于空白对照组(P<0. 05); MDA [ (5. 59±0. 33) nmol/ mg] 高于空白对照组(P<0. 05); 肝脏指数[ (4. 7±1. 5)%] 高于空白对照组(P<0. 05)。病理切片显示肝细胞坏死并出现脂肪空泡、纤维组织增生和炎细胞浸润。软肝冲剂低剂量组与秋水仙碱组治疗效果相似。软肝冲剂高剂量组效果较好, 小鼠血清中HA、LN、PCⅢ、Ⅳ⁃C、ALT 和AST 含量分别为(143. 21±21. 53) μg/ L、(139. 11±35. 23) μg/ L、(134. 45±24. 77) μg/ L、(119� . 32±29� . 34) μg/ L、(45� . 4±6� . 3) U/ L 和(53. 6±7. 1) U/ L, 均明显低于模型组(P<0. 05); 肝脏组织中SOD [ (189. 5±7. 5) U/ mg] 高于模型组(P<0. 05), MDA [ (2. 45±0. 33) nmol/mg] 低于模型组(P<0. 05); 肝脏指数[ (3. 4±0. 4)%] 低于模型组(P<0. 05); 病理检查显示肝脏组织中无炎性浸润、脂肪空泡和无纤维组织沉积。结论 软肝冲剂可以改善肝脏功能, 保护肝细胞和抑制肝纤维化, 减轻CCl4 诱导小鼠肝纤维化程度。 |
关键词: 软肝冲剂 肝纤维化 超氧化物歧化酶(SOD) 丙二醛(MDA) |
中图分类号: R99; O622.2
文献标识码:
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基金项目: |
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Protection effect of Ruangan Granule on carbon tetrachloride⁃induced liver damage in mice |
Wang Liangjun, Yang Liang
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Department of Hygiene, Liaoning Medical University, Jinzhou 121001, China
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Abstract: Objective To observe the protection effect or Ruangan Granule (RGG) on carbon tetrachloride induced⁃liverfibrosis in mice. Methods 60 C57B/ L mice were randomly divided into five groups including control group, model group, col⁃chicine group, RGG low dose group and RGG high dose group, with 12 mice in each group. Mice in control group were intrap⁃eritoneally injected with 100% peanut oil 1 ml/ kg twice a week for 10 weeks; mice in rest groups were all intraperitoneally injected with peanut oil containing 20% carbon tetrachloride 1 ml/ kg, twice a week, then colchicines [0. 1 mg/ (kg•d)], RGG[1 g/ (kg•d)]and RGG[5 g/ (kg•d)]were intragastrically administered for mice in colchicine group, low dose RGG group and high dose RGG group, respectively from third week. At the tenth week, the administrations were ended and the blood samples were collected by removing eyes method, and the contents of hyaluronic acid (HA), laminin (LN), three former Ⅲ (PCⅢ), collagen⁃Ⅳ (Ⅳ⁃C), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were measured, meanwhile, the contents of super oxide dismutase (SOD) and malondialdehyde (MDA) in liver tissue were also tested,the liver index was calculated and pathological changes of liver were observed by HE staining as well. Results The results showed that in model group, the values of HA, LN, PCⅢ, Ⅳ⁃C, ALT and AST in serum were (480. 11±62. 12) μg/ L、(411. 65±30. 63) μg/ L、(454. 12±41. 81) μg/ L、(311. 15±31. 61) μg/ L、(140. 4±9. 8) U/ L and (198. 2±9. 7) U/ L,respectively, significantly higher than those in other groups (P<0. 05), the SOD level in liver tissue (122. 53±7. 68) U/ mg,was significantly lower than those in other groups (P<0. 05), the MDA level in liver tissue (5. 59±0. 33) nmol/ mg, was significantly higher than that in control group (P<0. 05), the liver index (4. 7±1. 5)%, was significantly higher than that in con⁃trols group (P<0. 05), pathological examination clearly showed the necrosis, fat vacuoles of liver cells, fibrous tissue hyperplasia and inflammatory cell infiltration in liver. It also showed that the therapeutic effect of low dose RGG was similar to colchicines, the effect of high dose RGG seemed better: the values of HA, LN, PCⅢ, Ⅳ⁃C, ALT and AST in the serum of mice from hign dose group were (143. 21±21. 53) μg/ L、(139. 11±35. 23) μg/ L、(134. 45±24. 77) μg/ L、(119. 3±29. 34)μg/ L、(45. 4±6. 3) U/ L and (53. 6±7. 1) U/ L, respectively, were significantly lower than those in model group (P <0. 05); the SOD level in liver tissue of hign dose group was (189. 5±7. 5) U/ mg, significantly higher than that in model group(P<0. 05); the value of MDA in liver tissue of hign dose group was (2. 45±0. 33) nmol/ mg, significantly lower than that inmodel group (P<0. 05); liver index was (3. 4±0. 4)%, also significantly lower than that in model group (P<0. 05); pathological examination showed as well that there was no inflammatory infiltrates, fat vacuoles, and fiber deposition. Conclusion RGG may improve hepatic function, inhibit hepatic fibrosis, thereby can protect mice from hepatic injury by carbon tetrachloride. |
Keywords: Ruangan granule liver fibrosis superoxide dismutase (SOD) malondialdehyde (MDA) |