摘 要: 探究纳米二氧化硅(SiNPs)对淋巴管内皮细胞(HLECs)增殖、迁移及管状结构形成能力的影响及其 作用机制。 方法 采用 CCK⁃8 法检测 HLECs 增殖能力, Transwell 迁移小室法和基质胶法检测 HLECs 迁移和管状结构 形成能力, 并用 ELISA 实验检测细胞培养液中血管内皮生长因子⁃C (VEGF⁃C) 和血管内皮生长因子受体⁃3 (VEGFR⁃3) 的表达情况。 结果 在 10~ 40 μg / ml 浓度范围内, 随着 SiNPs 浓度的增加和作用时间的延长, HLECs 的增殖能力 受到抑制, 细胞培养液中 VEGF⁃C 的表达量逐渐降低, 与对照组相比差异有统计学意义 (P<0.05)。 不同浓度 SiNPs 作用后, 各组 HLECs 的迁移能力和管状结构形成能力均增强, 细胞培养液中 VEGFR⁃3 的表达量逐渐升高, 与对照组 相比差异有统计学意义(P<0. 05)。 结论 SiNPs 可能通过抑制 VEGF⁃C 的表达抑制 HLECs 的增殖, 增强 VEGFR⁃3 的 表达而促进其迁移和管状结构生成. |
关键词: 纳米二氧化硅 淋巴管增生 血管内皮生长因子⁃C (VEGF⁃C) 血管内皮生长因子受体⁃3 (VEGFR⁃3) |
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基金项目: 国家自然科学基金面上项目(编号:81773373) |
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Effect of nano⁃silica on proliferation, migration and tube⁃like structure formation of lymphatic endothelial cells |
XIU Lin⁃qing, YUN Lu, PAN Li, ZHANG
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(Qingdao University, Qingdao 266071, China)
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Abstract: To investigate the effects of nano⁃silica (SiNPs) on the proliferation, migration and tubular formation ability of human lymphatic endothelial cells (HLECs), and its mechanisms. Methods The proliferation of HLECs was detected by CCK⁃8 method, its migration and tubular structure formation abilities were detected by Transwell migration chamber method and Matrigel method, Elisa assay was used to detect the expression of vascular endothelial growth factor⁃C (VEGF⁃C)and vascular endothelial growth factor receptor⁃3 (VEGFR⁃3) in cell culture medium. Results It was found that in the range of 10~40 μg/ ml, with the increase of SiNPs concentration and the prolongation of acting time, the proliferative capacity of HLECs was inhibited, the expression of VEGF⁃C in cell culture medium also gradually decreased (P<0.05); while its migration and tubular formation abilities were obviously increased after incubated with SiNPs, and the expression of VEGFR⁃3 in cell culture medium were increased gradually as well (P<0. 05). Conclusion The results suggested that SiNPs may inhibit the proliferation of HLECs by inhibiting the expression of VEGF⁃C, and promote the migration and tubular structure by promoting the expressionof VEGFR⁃3. |
Keywords: nano⁃silica lymphatic hyperplasia vascular endothelial growth factor⁃C ( VEGF⁃C) vascular endothelial growth factor receptor⁃3 (VEGFR⁃3) |