摘 要: 目的探讨壹期矽肺患者血清中8-异前列腺素F2α(8-iso-PGF2α)、总抗氧化能力(T-AOC)和巨噬细胞衍生趋化因子(MDC)水平变化及意义。方法采用酶联免疫吸附法检测58例壹期矽肺(矽肺组)、35例具有与矽肺组相同接尘条件的健康井下接尘矿工(接尘组)和33例非接尘井上健康人员(对照组)血清中8-iso-PGF2α水平;用Luminex流式荧光技术检测血清MDC水平;化学比色法检测血清T-AOC水平。结果与对照组比较,接尘和矽肺组血清T-AOC水平均明显升高,接尘组MDC水平则明显降低,差异均具有统计学意义(P<0.01);与接尘组比较,矽肺组血清8-iso-PGF2α和T-AOC水平降低,MDC水平升高,差异也具有统计学意义(P<0.05,P<0.01);矽肺并发慢性阻塞性肺疾病(COPD)患者血清T-AOC水平高于单纯矽肺患者(P<0.05);饮酒矽肺患者血清8-iso-PGF2α水平低于非饮酒矽肺患者,差异具有统计学意义(P<0.05);壹期矽肺患者血清T-AOC与MDC呈负相关(r=-0.247,P<0.05)。结论壹期矽肺患者血清8-iso-PGF2α、T-AOC及MDC水平异常,并与矽肺发生发展有关;饮酒可影响壹期矽肺患者血清8-iso-PGF2α水平 |
关键词: 矽肺 8-异前列腺素F2α(8-iso-PGF2α) 总抗氧化能力(T-AOC) 巨噬细胞衍生趋化因子(MDC) |
中图分类号: R135.2
文献标识码:
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Serum levels of8-iso-PGF2α,T-AOC and MDC in stage I silicosis patients |
WANG Dong mei,YUAN Bao jun,TONG Yan yan,LI Chao,ZOu Ji min,GAO Li chang
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Department of Clinical Laboratory,Kai Luan General Hospital,Tangshan 063000,China
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Abstract: To explore the changes and the significance of serum levels of 8-iso-PGF2α,total antioxidant capacity(T-AOC)and macrophage derived chemokine(MDC)in stage I silicosis patients. Methods The levels of serum8-iso-PGF2αin fifty eight stage I silicosis patients(silicosis group),35 healthy miners(dust-contacted group)and 33 cases of healthy non underground workers(control group)were detected by enzyme inked immunosorbent assays,meanwhile,the MDC and T_OC levels were also detected by Luminex fluorescence technique and chemistry colorimentric method,respectively. esults omparing with control group,the serum T-C level in dust ontacted group and silicosis group was significantly increased,the serum MDC level in dust contacted group was significantly reduced(all P<0.01);the serum levels of8-iso-PGF2α and T-AOC were significantly lower and the serum levels of MDC in silicosis group was significantly higher than those of dust-contacted group(P<0.05,P<0.01)and there was a negative correlation between T-AOC and MDC in stage I silicosis patients( r =-0.247, P<0.05);additionally,serum levels of T-AOC in silicosis with COPD was significantly higher than that of simple silicosis(P<0.05);the levels of 8-iso-PGF2α in drinking silicosis patients were significantly lower than that of non-drinking patients(P<0.05). Conclusion The results suggested that in stage I silicosis patients,serum levels of8-iso-PGF2α,T-AOC and MDC were abnormal and related to the development of silicosis;and drinking might affect the serum level of 8-iso-PGF2α in silicosis patients. |
Keywords: silicosis 8-iso-prostaglandin-F2α(8-iso-PGF2α) total antioxidant capacity(T-AOC) macrophage derived chemokine(MDC) |