摘 要: 目的探讨壹期煤工尘肺(CWP)患者血清趋化因子γ-干扰素诱导蛋白10(IP-10)、巨噬细胞炎症蛋白-1α(MIP-1α)、巨噬细胞炎症蛋白-1β(MIP-1β)和巨噬细胞炎症蛋白-3α(MIP-3α)水平变化及意义。方法用Luminex流式荧光技术检测85例壹期CWP患者(CWP组)、44例井下健康工人(接尘对照组)及42例井上健康工人(正常对照组)血清IP-10、MIP-1α、MIP-1β和MIP-3α水平。结果与正常对照组比较,CWP组IP-10升高、MIP-1α降低,接尘组IP-10、MIP-1α和MIP-1β均降低,差异均有统计学意义(P<0.01,P<0.05);CWP组IP-10、MIP-1α、MIP-1β和MIP-3α水平均明显高于接尘对照组,差异具有统计学意义(P<0.01,P<0.05);与单纯CWP比较,CWP合并肺气肿患者MIP-3α水平下降,差异具有统计学意义(P<0.05)。CWP患者血清IP-10与MIP-1α、MIP-1β、MIP-3α以及MIP-1α和MIP-1β呈轻度正相关(rs=0.452、0.363、0.255、0.501,P<0.05)。结论壹期CWP患者血清趋化因子IP-10、MIP-1α、MIP-1β和MIP-3α水平异常,与尘肺发生发展有关;检测MIP-3α水平可能反映CWP病情程度。 |
关键词: 煤工尘肺 γ-干扰素诱导蛋白10 巨噬细胞炎症蛋白-1α 巨噬细胞炎症蛋白-1β 巨噬细胞炎症蛋白-3α |
中图分类号: R135.2
文献标识码:
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Serum levels of chemokines in stage Ⅰ coal workers’ pneumoconiosis and clinical significance |
YUAN Bao jun,LI Chao,WANG Dong mei,ZOU Ji min,GAO Li chang,TONG Yan yan
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Department of Clinical Laboratory,Kai Luan General Hospital,Tangshan 063000,China
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Abstract: To explore the serum levels of chemokines such as interferon γ induced protein 10(IP-
10),macrophage inflammatory protein-1α(MIP-1α),macrophage inflammatory protein-1β(MIP-1β) and macrophage inflamma-tory protein-3α(MIP-3α)in stage Ⅰ coal workers’ pneumoconiosis (CWP)and their clinical significance. Methods Serum levels of those chemokines mentioned above of 85 CWP patients(CWP group),44 healthy underground coal workers(dust-exposed control group)and 42 healthy ground workers(normal control group)were determined by a Multifunction Flow Fluorescence System Luminex 200. Results The results showed that compared with normal control group,the serum level of IP-10 in CWP group were significantly increased,whereas MIP-1α were decreased(P<0.01,P<0.05);serum levels of IP-10,MIP-1α and MIP-1β in dust-exposed control group were significantly lowered(P<0.01,P<0.05);while the serum levels of IP-10,MIP-1α,MIP-1β and MIP-3α in CWP group were significantly lower than those in dust-exposed control group(P<0.01,P<0.05),serum MIP-3α level in CWP patients with emphysema were significantly lower than those CWP patients( P<0.05).Additionally in CWP group,there were some slight positive correlations between IP-10 and MIP-1α,IP-10 and MIP-1β,IP-10 and MIP-3α,MIP-1α and MIP-1β(rs=0.452、0.363、0.255、0.501,P<0.05). Conclusion The serum levels of IP-10,MIP-1α,MIP-1β and MIP-3α in stage Ⅰ CWP group were all abnormal, which associated with the occurrence and development of CWP,and the detection of serum MIP-3α levels reflect the severity of CWP. |
Keywords: coal workers’ pneumoconiosis interferonγinduced protein 10(IP-10) macrophage inflammatory protein-1α(MIP-1α) macrophage inflammatory protein-1β(MIP-1β) macrophage inflammatory protein-3α(MIP-3α) |