摘 要: 目的筛选汞作业工人接汞早期血浆特异性microRNA(miRNA)分子。方法将研究对象分为汞吸收组和对照组,每组10人。先使用miRNA微阵列芯片分析两组miRNA表达谱,再对候选差异miRNA进行Real-Time PCR验证。结果在10个符合候选条件的microRNA中,最终选出2个miRNA:hsa-miR-16(P<0.001)、hsa-miR-451a(P<0.05),其在汞吸收组中相较于对照组显著下调,结果与芯片分析一致。结论 miR-16和miR-451a可能成为汞作业工人早期特异性分子标志物,并且可能在表观遗传水平上参与了汞在人体内的毒作用过程,但需要进一步探讨研究miRNA改变与汞暴露的因果关系 |
关键词: 汞 小分子RNA(miRNA) 血浆 |
中图分类号: R135.13
文献标识码:
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基金项目: 江苏省医学创新团队与领军人才项目(LJ201130) |
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Study on differential expression of microRNA in plasma of mercury workers |
DING Enmin,ZHAO Qiuni,PAN Liping,LIU Qingdong,WANG Boshen,BAI Ying,ZHU Baoli
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School of Public Health,Nanjing Medical University,Nanjing 211166,China
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Abstract: To identify mercury elated plasma microRNAs(miRNA)of workers during early stage of occupational exposure to mercury. Methods he subjects were divided into mercury absorption group and control group,each group had 10 individuals.In the discovery stage,microarray assay was used to detect the microRNA expression profiles in plasma samples from both groups.Then,the candidate differential miRNAs were validated by quantitative Real Time PCR. Results In 10 candidate miRNAs, only miR 16(P<0.01) and miR451a(P<0.05) were selected ,which significantly lower in mercury absorption group,consistent with microarray analysis. Conclusion The results suggested that the plasma miR16 and miR451a might be the potential biomarkers for excessive mercury exposure,these two plasma miRNAs may even involved in the toxicity mechanism of mercury at the epigenetics level.However, further studies are still necessary to prove the causal relationship between miRNAs and mercury exposure. |
Keywords: mercury microRNA(miRNA) plasma |