摘 要: 目的 探讨RAD52、XRCC2、XRCC4 基因多态性与1,3⁃丁二烯(BD) 作业工人DNA 修复能力的关系。方法 利用染色体断裂试验评价60 名BD 职业暴露工人和60名非暴露工人的外周血淋巴细胞对诱变剂博莱霉素(BLM) 所致DNA 损伤的修复能力, 聚合酶链反应⁃限制性片段长度多态性方法分析RAD52、XRCC2 和XRCC4 基因多态性。结果 职业BD 暴露组染色体断裂率[ (1.06±0. 41)%]显著高于对照组[ (0.85±0.36)%] (P<0. 01); 暴露组中XRCC4 A245G位点AA 基因型个体b/c 率[(1.18± 0.48)%] 显著高于GG或AG+GG 基因型个体[(0. 91±0. 29)%] (P =0. 02); XRCC4 T1394G 位点TT 基因型个体b/c 率[(0.75±0. 36)%]显著低于GG基因型个体[(1.13±0.42)%] (P =0.04)。结论 XRCC4基因多态性与BD作业工人DNA 修复能力存在相关性。 |
关键词: 1,3⁃丁二烯 基因多态性 DNA 修复能力 博莱霉素 |
中图分类号: R99; O623.122
文献标识码:
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基金项目: 唐山市科技支撑计划项目(No. 13130297z) |
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DNA repair capacity and gene polymorphism of DNA double strand break repair pathway in 1,3⁃butadiene workers |
Liu Nan, LI Bin, Cheng Juan, Li Yun, Zheng Guoying, Guan Weijun
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School of Public Health, North China University of Science and Technology; Key Laboratory of Coal Mine Health and Safety of Hebei Province, Tangshan 063000, China
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Abstract: Objective To explore the relation between genetic polymorphisms of RAD52, XRCC2, XRCC4 and DNA repair capacity (DRC) in 1,3⁃butadiene (BD) workers. Methods DRC of DNA damage caused by bleomycin in peripheral blood lymphocytes of 60 BD⁃exposed workers and 60 non⁃BD exposed workers were measured respectively, using chromatid breaks test, and the polymorphism of RAD52, XRCC2, XRCC4 were detected by polymerase chain reaction⁃restriction fragment length polymorphism (PCR⁃RFLP) technique. Results The results showed that the Chromosome breakage rate per cell (b/ c)were significantly higher in BD⁃exposed workers than that in control group[(1. 06±0. 41)% vs (0. 85±0. 36)%, P<0. 01],and the levels of b/ c were significantly lower in BD⁃exposed workers with AG+GG genotype of A245G polymorphism of XRCC4 than those with AA genotype[(0. 91±0. 29)% vs (1. 18±0. 48)%, P =0. 02], while the levels of b/ c[(1. 13±0. 42)%] in GG genotype of XRCC4 T1394G polymorphism were significantly higher than that of the TT genotype [ (0. . 75±0. 36)%, P =0. 04]. Conclusion The results suggested that there could be a correlation between polymorphisms of XRCC4 and DRC of DNA damage induced by BD. |
Keywords: 1,3⁃butadien genetic polymorphism DNA repair capacity bleomycin |