摘 要: 目的 通过人群研究和SNP功能分析,探讨对氧磷酶1(PON1)基因3’UTR区SNPs对miRNA结合的影响,为铅中毒对心血管系统产生危害的机制提供新的思路。方法ELISA方法检测铅中毒组和对照组中血清PON1含量,分析其在不同血铅浓度人群中的变化情况;Taqman MGB探针检测PON1基因rs854550和rs854552基因型分布情况;荧光素酶双报告基因法检测has-miR-218与rs854552所在区域结合情况。结果 低浓度血铅值的个体PON1血清水平显著下降(P< 0.05);rs854550和rs854552多态性位点在铅中毒组和对照组中未发现显著差异;报告基因实验也未能发现hsa-miR-218可能受rs854552影响其与3’UTR结合。结论 铅暴露能引起人体PON1表达改变,但本研究尚不能证明PON1基因3’UTR区多态性在铅中毒所致心血管疾病中的作用,需进一步实验寻找相关易感SNP,为明确铅中毒分子机制提供依据。 |
关键词: 铅中毒 单核苷酸多态性(SNP) 对氧磷酶1(PON1) miRNA |
中图分类号: R135.8
文献标识码:
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基金项目: 江苏省自然科学基金(BK20130112);国家自然科学基金(编号:81302409) |
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The study of association between SNPs in miRNA target region of PON1 and occupational lead poisoning |
Li Chunping, Wang Miaomiao, Wang Yiqing, Zhang Jinlong, Sun Na
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Department of Occupational Health, Wuxi Center for Disease Control and Prevention, Wuxi 214013, China
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Abstract: Objective To detect the association between SNPs in 3’UTR of PON1 and miRNA by analyzing population data and function of SNP, and provide a new direction of the mechanism of lead poisoning on the cardiovascular system. Methods We detected the serum PON1 levels in lead poisoning population and control population by ELISA; Genotypes distribution of rs854550 and rs854552 by Taqman MGB; association between hsa-miR-218 and rs854552 by Luciferase reporter gene assay. Results Individuals with low blood lead level had a significant decrease in serum PON1 level (P < 0.05); no significant difference between lead poisoning and control group was found in rs854550 and rs854552 genotypes; and no influence from rs854552 in the combination of has-miR-218 and 3’UTR. Conclusion Lead exposure can change PON1 level, however, it is not yet clear whether SNPs in 3’UTR of PON1 gene play a role in lead poisoning. Further studies are needed to find related susceptible SNPs, and to provide new evidence for the mechanisms of lead poisoning. |
Keywords: lead poisoning single nucleotide polymorphism (SNP) paraoxonase 1 (PON1)
miRNA |