摘 要: 目的 通过检测不同期别矽肺患者和接尘工人痰液(MCP-1)、(MIP-1α)含量变化及与肺通气功能相关性,探讨两种趋化因子与矽肺发病的关系。方法 将不同期别矽肺患者及有无接尘工人分为5组;用痰液诱导(sputum-induction, SI)的方法获取标本;以酶联免疫吸附方法测定痰中MCP-1、MIP-1α的含量。结果 MIP-1α及MCP-1组间差异均存在统计学意义(F=3.111, P=0.016;F=6.865,P=0.000)。MIP-1α与肺功能指标FEV1、FEV1/VCmax、MEF50、MEF25均呈明显正相关关系(P<0.05)。结论 MCP-1、MIP-1α可作为反映矽肺病程进展的敏感性生物指标,MIP-1α的表达水平更具备临床应用价值。 |
关键词: 矽肺 痰液诱导 趋化因子 单核细胞炎性蛋白-1(MCP-1) 巨噬细胞趋化蛋白-1α(MIP-1α) |
中图分类号: R135.2
文献标识码:
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基金项目: |
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A feasibility study of MCP⁃1 and MIP⁃1α as diagnostic biomarkers for silicosis |
Li Jinmu, Wang Rui, Sun Yinyin , Liu Xiuling
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University of Jinan; College of Medicine and Life Science, Shandong Provicaial Academy of Medical Sciences, Jinan 250062, China
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Abstract: Objective Through the detection of different stages of silicosis patients and workers exposed to dust the induced sputum MCP-1, MIP-1 alpha levels and correlation with pulmonary ventilation function of two kinds of trend factor and silicosis development. Methods Selection of different stages of silicosis patients and whether exposed workers were divided into five groups;Use induced sputum (Sputum-Induction, SI) method to obtain the object of study in sputum samples; Enzyme linked immunosorbent assay determination of content of sputum MCP-1, MIP-1 alpha. Results There were statistically significant differences between the groups in the MIP-1 group (F=3.111, P=0.016); There were statistically significant differences between the MCP-1 groups (F=6.865, P=0.000); The control group and other groups had significant differences and MIP-1 alpha and pulmonary function indexes FEV1, FEV1/VCmax, MEF50, MEF25 all showed a significant positive correlation (P<0.05). Conclusion The expression level of MIP-1 and MCP-1 can be used as a sensitive index to reflect the development process of the development of the lung disease. The expression level of MIP-1 has more clinical significance. randomly divided into control group and three MnCl2-exposed groups,twelve mice in each group,half male and half female. The control mice were treated with 0.9% NaCl, the Mn-exposed mice were treated with 12.5mg/kg,25mg/kg and 50 mg/kg MnCl2, respectively through intraperitoneal injection, once a day for two weeks. Then conduct the Morris water maze test and passive avoidance test, 24h after last passive avoidance test, all mice were sacrificed by decapitation after anesthesia and take the striatum. The morphological and ultrastrutural changes of striatum were observed by light and electron microscopes, meantime, the activities and protein levels of NR2A and NR2B were detected as well with immunohistochemical and Western blotting techniques. Results The results showed that at the later stage of Morris water maze test, the escape latent duration and swimming distance of mice in 50mg/kg MnCl2 group had some increase compared with that of control group, but at the 6th day and 13th day after administration, the frequency of crossing loop in mice of 50mg/kg MnCl2 group was obvious decreased as well as the latent duration of entering dark room after 24h of MnCl2 exposure, but the frequency of entering dark room was obviously increased. With the increase of Mn doses, there were some changes occurred both in the pathological structures and ultrastructures in striatums of mice, and the positive area and IOD (integral optical density) of the immunohistochemical expression of NR2A and NR2B as well the protein expression levels were all gone down. Conclusion The results showed that manganese may result in learning and memory dysfunction via disturbing the NMDAR of striatum. |
Keywords: silicosis sputum induction chemokine MCP-1 MIP-1α |